The seeds of Acampe praemorsa (Roxb.) Blatt. & McCann successfully germinated in vitro on 0.8% (w/v) agar solidified KC, MS, MVW,
and PM basal media and developed into seedlings. Both in vitro raised seedlings and in vivo obtained plant parts (leaf, stem, and root) were
investigated for antioxidant, anti-inflammatory, and cytotoxic activities. The moderate antioxidant activity was showed in methanolic crude
extract of leaf (IC50= 229.55 μgml-1), stem (IC50= 103.86 μgml-1), root (IC50= 222.22 μgml-1), and in vitro seedlings (IC50= 208.47 μgml-1)
samples. Strong anti-inflammatory activity was observed on leaf (IC50= 95.15 μgml-1), stem (IC50= 63.87 μgml-1), and in vitro developed
seedlings (IC50= 80.39 μgml-1), whereas root sample showed the moderate (IC50= 137.50 μgml-1) anti-inflammatory activity. The cytotoxic
activity of methanolic crude extract of leaf, stem, root, and in vitro raised seedlings exhibited moderate activity with the LC50 values as
265.20, 224.13, 357.73, and 160.21 μgml-1, respectively.